Agricultural Science&Technology

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农业基础科学与方法

  • Hapseeker:一个分析单倍型的C++程序(英文)

    谌平;郑伟;王留阳;

    The C++ program: Hapseeker was developed to analyze DNA or RNA sequence, besides, Hapseeker could be used to identify haplotype, calculate frequency of each haplotype as well as find variable site quickly. Moreover, Hapseeker had many advantages such as simple operation, rapid running speed and high accuracy.

    2009年03期 v.10 1-2+6页 [查看摘要][在线阅读][下载 142K]
    [下载次数:26 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:7 ]
  • 3,13-酯基中乌头碱衍生物的合成及其杀虫活性的研究(英文)

    王晓东;袁小红;杨海君;侯大斌;王惠;许源;

    [Objective] The experiment aimed to explore the relations between structure of aconitine, aconitine derivatives and insecticide. [Method] The aconitine derivatives such as 3-acetyl mesaconitine, 3-propionyl mesaconitine, 3-acetyl-13-Benzoyl-mesaconitine and 3-propionyl-13-Benzoyl-mesaconitine were synthesized by mesaconitine with acetic anhydride or propionic anhydride and their insecticidal activities were also determined. [Result] When the concentration was 500 mg/L, the insecticidal activities of mesaconitine against Nilaparvata legen and Aphis were 50% and 30% respectively while the insecticidal activities of 3-acetyl mesaconitine, 3-propionyl mesaconitine, 3-acetyl-13-Benzoyl-mesaconitine and 3-propionyl-13-Benzoyl-mesaconitine against Aphis medicagini were 40%, 30%, 30% and 20% respectively at 500 mg/L. [Conclusion] After hydroxyl esterification, the insecticidal activity of mesaconitine was declined and the existence of hydroxyl at 3rd position in mesaconitine played very important influences on insecticidal activity.

    2009年03期 v.10 3-6页 [查看摘要][在线阅读][下载 305K]
    [下载次数:70 ] |[网刊下载次数:0 ] |[引用频次:7 ] |[阅读次数:5 ]
  • 一种新的非酶法分离荠幼胚的方法(英文)

    洪亚平;张亚冰;吴国锋;赵文娟;阎婵婵;

    [Objective] The aim of this study was to manually isolate the microstructures such as ovule and embryo of Capsella bursa-pastoris (L.) Medic. by the non-enzymatic method. [Method] With the gum block and dissecting microscope, its floral characteristics, ovule and embryo were manually isolated and observed by the non-enzymatic method. [Result] C. bursa-pastoris had four calyces, four petals, tetradynamous stamen and silicle, while its embryogenesis contained globular embryo, heart-shaped embryo and torpedo-shaped embryo. [Conclusion] The microstructures such as ovule and embryo of plants become easier to be isolated manually with the viscosity of gum block, which has small damage on embryo or disadvantages of keeping original shapes, and also can used in multi fields studies. This test can also be accomplished in labs with poor experiment facilities.

    2009年03期 v.10 36-38页 [查看摘要][在线阅读][下载 296K]
    [下载次数:25 ] |[网刊下载次数:0 ] |[引用频次:4 ] |[阅读次数:4 ]
  • 杜鹃花色素的提取及稳定性研究(英文)

    罗向东;谢建坤;张乐华;戴亮芳;

    [Objective] The aim of this study was to further investigate and utilize the natural anthocyanidin from Rhododendron L. [Method] Using Rhododendron L. with four different colors including white, pink, red and purple as the test materials, its anthocyanidin extraction methods were analyzed in this study to evaluate the spectral properties and its stability of various anthocyanidin. [Result] The anthocyanidin from Rhododendron L. was perfectly extracted by methanol with 1% concentrated hydrochloric acid (V/V) and had better stability in this extraction solution. The further experiment in vitro indicated that the anthocyanidin from Rhododendron L. became stable with pH value of 0-3, but could not resistant to high temperature or strong light, and the alkaline condition had also great effects on its stability. [Conclusion] The methanol with 1% concentrated hydrochloric acid (V/V) has the best effect for extraction, and the anthocyanidin from Rhododendron L. is more stable in low temperature, weak light and acid conditions.

    2009年03期 v.10 139-143页 [查看摘要][在线阅读][下载 618K]
    [下载次数:206 ] |[网刊下载次数:0 ] |[引用频次:19 ] |[阅读次数:7 ]
  • 水溶性壳聚糖纳米载体蛋白药物的体外释放模型(英文)

    王春;孙胜玲;肖惠宁;何北海;杨连生;

    [Objective] The experiment aimed to explore release rule of water-soluble chitosan (WSC) in vitro. [Method]The bovine serum albumin(BSA) was taken as a model protein drug and some existing release models such as Kinetics model, Gompertz model, Weibull model, Higuchi model and Logistic model were used to fit the BSA release profile from WSC carriers. [Result] Except Higuchi model and Logistic model, other models could fit BSA release profile better. [Conclusion] Gompertz two-order kinetics model could fit the release of WSC nano-particles better and model parameters had practical physical meaning.

    2009年03期 v.10 144-147页 [查看摘要][在线阅读][下载 465K]
    [下载次数:289 ] |[网刊下载次数:0 ] |[引用频次:10 ] |[阅读次数:4 ]

植物生理·生化

  • 土壤镉污染对大蒜生理生化指标的影响(英文)

    钱翌;杨立杰;

    [Objective] The experiment aimed to study the effects of cadmium pollution in soil on physiological and biochemical index of Allium sativum L. and provided reference for the recovery of cadmium pollution in soil. [Method]By setting eleven Cd concentrations from 0.21 to 500 mg/kg in soil and the pot test, ecological corresponding mechanism of plant height, chlorophyll (Chl) content, catalase (CAT) activity and malondialdehyde (MDA) of Allium sativum L. was analyzed. [Result] The plant height had a strong tolerance to cadmium pollution in soil, while the total chlorophyll content and chlorophyll a content had no significant difference compared with control treatment, except Cd concentration was 500 mg/kg. The high Cd concentration would increase the damage to membrane of Allium sativum L. however with the regulation of physiological mechanism, the damage was gradually decreased.[Conclusion] Allium sativum L. had strong eco-physiological adaptability to Cd contaminated soil and it had potential for recovering Cd contaminated soil.

    2009年03期 v.10 7-10页 [查看摘要][在线阅读][下载 255K]
    [下载次数:161 ] |[网刊下载次数:0 ] |[引用频次:28 ] |[阅读次数:32 ]

农业生物技术

  • 利用C基因组对高杆野生稻和宽叶野生稻基因组的比较分析(英文)

    刘凤麟;赵侯明;李刚;吴绮;覃瑞;刘虹;

    [Objective] Genomic in situ hybridization (GISH) was used to study the relationship between the two CCDD genomes of Oryza alta and Oryza latifolia. [Method] Total DNA of Oryza officinalis (C-genome) was used as a probe for genomic in situ hybridization on metaphase chromosomes from Oryza alta and Oryza latifolia, respectively. [Result] Under certain post-hybridization washing stringencies, C- and D-genome could be distinguished in CCDD genome type; there were huge differences in some CC chromosomes of Oryza alta, Oryza latifolia, and Oryza officinalis. The genome of Oryza latifolia was more original. [Conclusion] Comparative analysis of the Oryza species with identical genome type may facilitate to elucidate the possible approaches to plant genome evolution and species evolution.

    2009年03期 v.10 11-14+21页 [查看摘要][在线阅读][下载 413K]
    [下载次数:44 ] |[网刊下载次数:0 ] |[引用频次:6 ] |[阅读次数:10 ]
  • 表达小反刍兽疫病毒H基因的山羊痘病毒通用转移载体的构建及鉴定(英文)

    邵长春;张强;吴国华;颜新敏;李健;王建科;卢晓丽;赵志荀;崔丽凡;高世功;

    [Objective] This study was to develop a live vector vaccine of goat pox virus of Peste des petits ruminants(PPR). [Method] Using PCR amplification technique, PPR H gene was obtained, then ligated into pGEM-T easy vector; the recombinants were digested by Nhe Ⅰ and Hind Ⅲ, and ligated into pEGFP-N1-P7.5, yielding the recombinant vector pEGFP-N1-P7.5-H; next the expression cassette EGFP-N1-P7.5-H was first released from recombinant vector pEGFP-N1-P7.5-H by double digestion of Hind Ⅲ and Nhe Ⅰ and ligated into pUC119-TK that was digested by Kpn Ⅰ, yielding the transfer vector pUC119-TK-EGFP-P7.5-H. [Result] Identification and double enzyme digestion showed that the transfer vector pUC119-TK-EGFP-P7.5-H was correctly constructed. From the transfer vector transfected BHK-21 cells which infected GTPV AV41, specific fluorescence was observed at 48th h of transfection. [Conclusion] The construction of goat poxvirus live vector laid a foundation for the live vector vaccine of PPR vaccine.

    2009年03期 v.10 15-18+35页 [查看摘要][在线阅读][下载 429K]
    [下载次数:100 ] |[网刊下载次数:0 ] |[引用频次:3 ] |[阅读次数:7 ]
  • 一种高效提取棉花细胞核的技术(英文)

    彭仁海;刘方;宋国立;王春英;黎绍惠;张香娣;王玉红;王坤波;

    [Objective] The experiment aimed to study an efficient method of Nuclei extraction of cotton and provided technical support for constructing large-insert genomic library and sequencing complete genome. [Method] The cotton cotyledons germinated in dark moisture chamber for one week were chopped with a sharp sterile scalpel in a Petri dish which contained ice-cold nucleus isolation buffer (10 mmol/L MgSO4, 5 mmol/L KCl, 0.5 mmol/L HEPES, 1 mg/ml DTT, 0.25% Triton X-100 and 2% PVP40), then the nuclei were collected after selected through 100, 50 and 30 μm nylon meshes and centrifugation. Meanwhile, the tender leaves and cotyledons with different germination time in dark were treated by grinding method and sharp scalpel method. [Result] The chopping with a sharp scalpel method was very simple and rapid, which did not need grind and mercaptoethanol treatment and the successful extraction rate was 100%.[Conclusion] An efficient method of nuclei extraction of cotton with simple, high efficiency, rapid reaction and poison free were established.

    2009年03期 v.10 19-21页 [查看摘要][在线阅读][下载 216K]
    [下载次数:88 ] |[网刊下载次数:0 ] |[引用频次:3 ] |[阅读次数:6 ]
  • 麝致病性大肠杆菌的质粒DNA分析(英文)

    罗燕;程建国;郑士华;赵翠;李蓓;李敏;

    [Objective] The pathogenic Escherichia coli in musk deer was classified at molecular level to provide basic materials for molecular epidemiology of pathogenic Escherichia coli in musk deer. [Method] Plasmids from 24 pathogenic Escherichia coli in musk deer were extracted by the Lysis Triton method, and then identified by single enzyme digestion with three endonucleases of Hind Ⅲ, EcoR Ⅰ and BamH Ⅰ. [Result] The yield rate of plasmids was 91.6%, and 24 pathogenic Escherichia coli in musk deer had the identical or similar plasmid profiles. [Conclusion] Plasmid DNA analysis offers scientific basis for molecular epidemiology of pathogenic Escherichia coli in musk deer in Sichuan Institute of Musk Deer Breeding.

    2009年03期 v.10 22-25页 [查看摘要][在线阅读][下载 1033K]
    [下载次数:72 ] |[网刊下载次数:0 ] |[引用频次:9 ] |[阅读次数:6 ]
  • 雄性育性基因RNA干扰载体的构建与鉴定(英文)

    王文锋;王红霞;穆灵敏;

    cDNA fragment of fertility gene MS2 from cotton was cloned by RT-PCR approach, it was highly homologous with relevant genes of Brassica napus and Arabidopsis thaliana. According to the principles of constructing RNAi vector, sense and antisense fragments of MS2 gene carrying restriction endonuclease recognition sites were amplified via PCR technique, ligated with the first intron of upland cotton chinase gene, then inserted into artificially modified plant expression vector pBI121, yielding RNAi vector pBGP12MSIn. The results showed that RNAi vector pBGP12MSIn harboring MS2 gene driven by anther specific promoter BGP was successfully constructed. Our results laid a foundation for studying the function of this gene and genetic transformation of plant male sterile lines.

    2009年03期 v.10 26-28页 [查看摘要][在线阅读][下载 311K]
    [下载次数:68 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:8 ]
  • 大白菜基因组DNA的提取及AFLP反应体系的建立(英文)

    孟淑春;张海英;郑晓鹰;刘玉梅;王永健;

    [Objective] The obtained clear AFLP fingerprint of Chinese cabbage provided basis for studies on the molecular markers of Chinese cabbage cultivars and the phylogenetic relationship among Chinese cabbage cultivars. [Method] With the test materials of leaves of Chinese cabbages, the high-quality total DNA from leaves of Chinese cabbages was extracted by the modified CTAB method. DNA restriction-ligase reaction, pre-amplification and selective amplification were optimized, and the AFLP silver-staining reaction system for Chinese cabbage was established. [Result] The quality of DNA template influenced restriction enzyme digestion and the subsequent ligase amplification reaction, while the modified CTAB extraction method could be used in AFLP analysis of Chinese cabbage to obtain a clear AFLP fingerprint. The optimum conditions for restriction enzyme digestion of genomic DNA from Chinese cabbage were as follows: 150 g DNA template, 12.5 μl reaction volume, 1.25 U Eco R Ⅰ, 1.25 U Mse Ⅰ and 5×Reaction Buffer with 4 h at 37 ℃. The ligation reaction with 2.5 h at 20 ℃ was the optimum condition. Six pairs of primers including E-AAC/M-CAG, E-AAG/M-CAC, E-ACA/M-CTG, E-ACT/M-CAC, E-ACT/M-CTT and E-ACT/M-CTC all had its own stable and clear patterns. [Conclusion] With abundant bands and high polymorphism, AFLP selective amplification is an efficient molecular marker for genomic polymorphism of Chinese cabbage.

    2009年03期 v.10 29-35页 [查看摘要][在线阅读][下载 653K]
    [下载次数:147 ] |[网刊下载次数:0 ] |[引用频次:13 ] |[阅读次数:9 ]
  • 绿头鸭线粒体ND2基因全序列测定及其研究(英文)

    涂剑锋;司方方;邢秀梅;杨福合;

    [Objective] The study was to analyze the phylogenesis of Anas platyrhynchos. [Method] Complete sequence of mitochondrial ND2 gene of 4 Anas platyrhynchos was determined by direct DNA sequencing based on PCR products. Combined with ND2 gene sequences of the Anas Linnaeus accessed in GenBank, phylogenetic tree was constructed by Neighbor-joining and maximum parsimony methods. [Result] The ND2 gene sequences of 4 Anas platyrhynchos were identical(1 041 bp in length; the nucleotide contents of A, G, T, and C were 28.91%, 13.35%, 20.75% and 36.98% respectively; A+T content approximated to that of C+G). Sequences of ND2 gene of mallard were same as spotbill duck, and had high homology with others. The phylogenetic trees indicated mallard and spotbilled duck were close in genetic relationship, both shared a haplotype; then Philippine duck, green-winged teal and northern pintail fell into branch ''A". [Conclusion] The domestic duck may be domesticated from mallard and spotbilled duck.

    2009年03期 v.10 46-49页 [查看摘要][在线阅读][下载 500K]
    [下载次数:78 ] |[网刊下载次数:0 ] |[引用频次:5 ] |[阅读次数:5 ]
  • “大通”牦牛Lfcin基因克隆及生物信息学分析(英文)

    裴杰;阎萍;姬国红;冯瑞林;梁春年;郭宪;曾玉峰;包鹏甲;褚敏;

    [Objective] This study was to clone Lfcin gene from Datong yak, so as to provide reference for applying this gene in feed industry and breeding industry. [Method] Using PCR technology, the lactoferricin(Lfcin)-encoding gene was obtained from genome of Datong yak; then it was cloned into pGEM-T easy vector, and then sequenced; the sequencing results were subsequently aligned with the sequences of dairy cow accessed in GenBank. Moreover, amino acid sequences of Lfcin gene from various species including yak, dairy cow, human and mouse were used for sequence alignment and phylogenesis analysis. [Result] The second exon of lactoferrin(LF) from Datong yak, which is 778 bp in length, was obtained, within which the coding region of Lfcin gene is 75 bp (25 amino acid residues); sequence analysis showed that there is discrepancy of eleven bases between Datong yak and dairy cow; Lfcin proteins from various species shared high homeology, of which that from Datong yak and dairy cow were completely identical; phylogenesis analysis showed that cladogram based on Lfcin was consistent with species evolutionary law. [Conclusion] This study laid a foundation for the prokaryotic or eukaryotic expression of Lfcin gene and further understanding the activity of Lfcin protein.

    2009年03期 v.10 50-54页 [查看摘要][在线阅读][下载 769K]
    [下载次数:67 ] |[网刊下载次数:0 ] |[引用频次:4 ] |[阅读次数:5 ]
  • E.tenella吉林株单卵囊的分离及PCR鉴定(英文)

    杨桂连;李建华;张西臣;赵权;宫鹏涛;蔡亚南;张国才;

    [Objective] In order to get a purified strain to carry out the relative molecular biology research about E.tenella. [Method] The single-oocyst isolation method was improved and the isolated single-oocyst which was put into capsule was fed to chickens. At the same time, the collected oocysts were identified by PCR method. [Result] The oocysts were isolated from feces of 15 chickens among that of 20 chickens and the infection rate was 75%. The PCR results demonstrated that the single-oocyst strain was E.tenella. [Conclusion] The inoculation of single oocyst capsule was simple, besides, this method did not only save time but also declined inoculation difficulty, increased infection rate and provided good materials for biological research of coccidian.

    2009年03期 v.10 55-57+62页 [查看摘要][在线阅读][下载 310K]
    [下载次数:105 ] |[网刊下载次数:0 ] |[引用频次:15 ] |[阅读次数:7 ]
  • 拮抗链霉菌AFLP分析技术体系的研究(英文)

    金来武;刘伟成;潘争艳;裘季燕;刘学敏;

    [Objective] The aim of this study was to investigate the preparation method and amplification system of antagonistic streptomyces DNA templates based on AFLP assays, and also provide a basis for the application of AFLP technology in the analysis of streptomyces or even actinomyces. [Method] The DNAs were extracted by the modified CTAB method and amplified by the Pst Ⅰ/Mse Ⅰ AFLP kit and its reaction system. The amplified products were analyzed by the denatured polyacrylamide gel electrophoresis. [Result] The genomic DNAs of ten antagonistic strains of Streptomyces were extracted and tested. The result of 0.8% agarose gel electrophoresis showed that the major DNA bands were clear without degradation and RNA residue, with the fragment sizes ranging from 37.64 to 40.86 Kb. By ultraviolet spectrophotometry, the OD260/OD280 values varying from 1.625 to 1.833 were obtained. Furthermore, the agarose gel electrophoresis of DNA products digested by Pst Ⅰ/Mse Ⅰ presented the dispersed fluorescent long band, which indicated that the enzymatic hydrolysis was fully carried out. The amplified bands of DNA templates by the screened three pairs of primers were clear with rich polymorphism. [Conclusion] The preparation method and amplification system of DNA template established in this study can be used in the AFLP analysis of Streptomyces.

    2009年03期 v.10 58-62页 [查看摘要][在线阅读][下载 451K]
    [下载次数:31 ] |[网刊下载次数:0 ] |[引用频次:3 ] |[阅读次数:9 ]
  • 利用ISSR分子标记分析44份红麻种质资源的遗传多样性(英文)

    霍光;李德芳;陈安国;李建军;唐惠娟;

    [Objective] This study was to reveal the genetic diversity and genetic relationship of the kenaf(Hibiscus cannabinus L.) resources from different origins, thus providing basis for genetic improvement and molecular marker-assisted breeding of kenaf. [Method] Ninety one ISSR molecular markers were used for amplification on 44 shares of kenaf germplasm resources, of which 21 showing good diversity and clear bands were chosen for PCR amplification. Based on amplification results, the genetic similarity coefficients among kenaf germplasm resources were calculated by using analytic software NTSYSpc-2.10e, and phylogenetic tree was then established via UPGMA. [Result] Totally 169 bands were amplified using the 21 screened primers, averagely 8.05 bands were amplified from each primer. Of them, 141 bands were polymorphic, accounting for 83.4%. When genetic similarity coefficient 0.887 was used as criterion L1, these 44 shares of kenaf germplasm could be classified to be 32 shares of cultivars and 12 shares of wild type or half-wild type varieties. When genetic similarity coefficient 0.897 was used as criterion L2, these 32 shares of cultivars could be further grouped into four sub-clusters. The genetic diversities between cultivars and wild type or half-wild type varieties were between 0.46-0.91, showing huge hereditary difference; while that among 32 cultivars were between 0.85-0.97, suggesting that genetic relationships among cultivars are relatively close and their genetic similarities are rather narrow. [Conclusion] ISSR could well determine the genetic similarities among kenaf germplasm resources and provide valuable molecular information for selecting parents of hybrid cross, which can lay a good foundation for DNA mapping of kenaf germplasm resources.

    2009年03期 v.10 63-67页 [查看摘要][在线阅读][下载 384K]
    [下载次数:128 ] |[网刊下载次数:0 ] |[引用频次:27 ] |[阅读次数:19 ]
  • 青海云杉ISSR-PCR反应体系的建立与优化(英文)

    王文峰;李毅;马彦军;李栋栋;

    [Objective] The experiment aimed to determine the optimum ISSR-PCR reaction system of Picea crassifolia kom. [Method] Picea crassifolia kom. was used as material to select and optimize influencing factors of ISSR-PCR such as Mg2+, dNTPs, Taq DNA polymerase, template DNA, primers, annealing temperature. [Result] The optimum ISSR-PCR reaction system in 20 μl reaction system was consisted of 1 μl 10×buffer, 1.5 mmol/L Mg2+, 0.2 mmol/L dNTPs, 1.0 U Taq DNA polymerase, 40 ng template DNA, 0.6 μmol/L primers. According to gradient test of annealing temperature in optimum ISSR-PCR reaction system of Picea crassifolia kom, it was found that the optimum annealing temperature of UBC 818 was 54.2 ℃ and the annealing temperature was different for different primers.[Conclusion]The construction of ISSR-PCR reaction system provided technical basis for classification of germplasm resources, construction of genetic map, gene mapping of Picea crassifolia kom. through using ISSR technology.

    2009年03期 v.10 68-71页 [查看摘要][在线阅读][下载 364K]
    [下载次数:82 ] |[网刊下载次数:0 ] |[引用频次:2 ] |[阅读次数:11 ]

动物科学 

  • 水牛子宫内膜腺上皮细胞和基质细胞的分离·培养和生物学特性研究(英文)

    庄新杰;段亚苹;黄怡;宁淑芳;张明;

    [Objective] The experiment aimed to set up a method for isolating and culturing endometrial stromal cells (BESC) and endometrial glandular epithelial cells(BEGEC) of buffalo as well as laid foundation for studying biological mechanism of embryo implantation and uterine diseases. [Method] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique were used to isolate BESC and BEGEC, then immunocytochemical method and TRYPAN-Blue assay were used to determine the purity and survival rate of isolated cells. [Result] The BESC and BEGEC were successfully isolated and cultured while immunocytochemical method and cell count method demonstrated that the purity was over 90%. The result of TRYPAN-Blue assay shown that survival rate of BESC and BEGEC was 91% and 78% respectively. [Conclusion] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique could isolate BESC and BEGEC with high purity.

    2009年03期 v.10 39-42+71页 [查看摘要][在线阅读][下载 435K]
    [下载次数:44 ] |[网刊下载次数:0 ] |[引用频次:7 ] |[阅读次数:9 ]

动物科学

  • 猪繁殖与呼吸综合症GP5蛋白的纯化与免疫活性分析(英文)

    陈妍;田宏;尹双辉;尚佑军;刘湘涛;

    [Objective] The purification and immunocompetence of GPS protein in porcine reproductive and respiratory syndrome (PRRS) were analyzed in this study, which provided basis for establishing the corresponding serological method. [Method] The recombinant expression plasmid pGEX-6P-5 was transformed into BL21 and expressed after being induced with IPTG. The solubility analysis of expression products was carried out, and then the recombinant protein was purified for SDS-PAGE identification and Western-blot analysis. Finally, the recombinant antigen was used in the immune experiment of guinea pigs. [Result] The target protein content accounted for 30% of the total cells protein content according to the chromatography scanning, and the purity of target protein after purification reached 80%. The purified protein was analyzed by Western-blot and immune experiment of guinea pigs, and the results showed that the expressed protein had good reactionogenicity and immunogenicity. [Conclusion] This study provides materials for further studies on the function between PRRSV ORF5 gene and its editing protein, which also lays a foundation for porcine reproductive and respiratory syndrome virus genetic engineering products.

    2009年03期 v.10 97-100页 [查看摘要][在线阅读][下载 331K]
    [下载次数:79 ] |[网刊下载次数:0 ] |[引用频次:4 ] |[阅读次数:8 ]
  • 美凤蝶非滞育蛹·滞育蛹与滞育蛹羽化成虫核酸含量比较(英文)

    易传辉;陈晓鸣;史军义;周成理;

    [Objective] The aim of this study was to provide basis for deeply understanding the diapause mechanism of Papilio memnon L. [Method] RNA and DNA content of non-diapause pupae, diapause pupae and eclosion-adult from diapause pupae at different development stages were detected by the colorimetry. [Result] RNA content of non-diapause pupae was 4.614 0-7.946 3 μg/mg, while diapause pupae was 4.326 0-5.885 3 μg/mg and eclosion-adult from diapause pupae was 20.779 3 μg/mg at initial stage. DNA content of non-diapause pupae was 0.448 7-0.535 0 μg/mg, while diapause pupae was 0.452 0-0.828 3 μg/mg and eclosion-adult from diapause pupae was 1.727 0 μg/mg at initial stage. [Conclusion] The nucleic acid content and change is related to the development stage.

    2009年03期 v.10 101-103+107页 [查看摘要][在线阅读][下载 237K]
    [下载次数:56 ] |[网刊下载次数:0 ] |[引用频次:2 ] |[阅读次数:8 ]
  • 利用Asia 1型口蹄疫病毒VP1蛋白的单克隆抗体建立单抗竞争ELISA方法(英文)

    林彤;邵军军;丛国正;独军政;高闪电;常惠芸;谢庆阁;

    Using the purified VP1 protein of Asia 1 type foot-and-mouth disease virus as the antigen, the purified monoclonal antibody was labeled by the sodium periodate method and the monoclonal antibody competitive ELISA was established in this study. Ten positive porcine foot-and-mouth disease serums and more than two hundreds negative serum were tested, and the results were the same as the background of samples. The sensitivity test and replicate test indicated that this method was stable and sensitive, which was suitable for monitoring Asia 1 type porcine foot-and-mouth disease virus antibody.

    2009年03期 v.10 104-107页 [查看摘要][在线阅读][下载 235K]
    [下载次数:121 ] |[网刊下载次数:0 ] |[引用频次:5 ] |[阅读次数:9 ]

园林·园艺

  • 极度濒危植物百山祖冷杉水培繁殖(英文)

    饶龙兵;吴友贵;

    [Objective] The experiment aimed to explore the influences of phytohormones (ABT and IAA) and nutrient solution on rooting of Abies beshanzuensis M.H.Wu by water cultured medium. [Method] The Abies beshanzuensis M.H.Wu were treated by water (CK), 10 mg/L ABT+ water, 10 mg/L IAA+ water, 10 mg/L ABT+ hoagland solution, 10 mg/L IAA+ hoagland solution, then the rooting process was observed and the formation rate of callus, rooting rate, number of rooting, and root length were investigated and analyzed. [Result] ABT and IAA had obvious influences on callus induction, rooting rate and the number of root of Abies beshanzuensis M.H.Wu by water culture, so they were suitable to be used in water propagation of Abies beshanzuensis M.H.Wu. The treatments of phytohormones had no regular influences on the longest root length and average root length. The nutrient solutions would not generate obvious influence on propagation of Abies beshanzuensis M.H.Wu at firstly stage, but they generated influence on root growth after rooting. [Conclusion] The research provided new ideas for propagation of Abies beshanzuensis M.H.Wu, which could make it out of endangerment situation quickly.

    2009年03期 v.10 43-45+54页 [查看摘要][在线阅读][下载 280K]
    [下载次数:106 ] |[网刊下载次数:0 ] |[引用频次:6 ] |[阅读次数:6 ]
  • 沙枣引种试栽研究(英文)

    王柏青;黎敏霞;王耀辉;

    [Objective] The experiment aimed to explore the possibility of introducing Elaeagnus angustifolia L. in saline land of West Jilin Province for improving local ecological environment. [Method] The Elaeagnus angustifolia L. from Ningxia Province were sown in nursery and practice base of Forestry College in Beihua University to observe their phenophase, growth state, stress resistance and study the relations between survival rate and different cutting medium, grafting methods. [Result] Elaeagnus angustifolia L. could adapt to climate condition in Jilin area and grow well . Among 3 cold-proof measures, there was no freezing injury by soil burial method, although there was 2.8% and 3.9% mortality by covering grassy marshland and winding straw, the cold injury degree was light, so they could go through winter normally. Meanwhile, Elaeagnus angustifolia L. had good saline-alkali tolerance, drought resistance and flood resistance. The successful cutting and graft of Elaeagnus angustifolia L. provided a condition for planting in large-scale and knew the rooting rate of different cutting medium. [Conclusion] Elaeagnus angustifolia L. could be introduced to plant in West Jilin Province.

    2009年03期 v.10 72-75+83页 [查看摘要][在线阅读][下载 275K]
    [下载次数:57 ] |[网刊下载次数:0 ] |[引用频次:6 ] |[阅读次数:7 ]
  • 孑遗植物银杏(Ginkgo biloba L.)雌雄株水分生理特征研究(英文)

    曹全;江洪;曾波;余树全;王彬;蒋馥蔚;

    [Objective] The experiment aimed to study the difference of water physiology of male and female Ginkgo biloba L. for discussing the strategy of water utilization as well as the important role of this difference during evolution process. [Method] The stem sap flow, stomatal conductance(Gs), transpiration rate(Tr) and water use efficiency (WUE) of male and female Ginkgo biloba L. were comparatively studied. [Result] The day-night processes of flow on male and female Ginkgo biloba L. were similar. The flow on male and female Ginkgo biloba L. in day were almost same while the flow at night on male Ginkgo biloba L. was bigger than that on female Ginkgo biloba L. The Tr and Gs of male and female Ginkgo biloba L. were high in morning and at night but low at noon ,while Tr and Gs of female Ginkgo biloba L. in morning and at night were higher than these of male Ginkgo biloba L. at the same time point. However, these indexes of female plant were lower than these of male plant from 11:00 to 14:00. WUE changing trends of male and female Ginkgo biloba L. were similar, while average water utilization rate of female Ginkgo biloba L. was slightly lower than that of male Ginkgo biloba L. [Conclusion] Compared with other companion plants, water physiology of male and female Ginkgo biloba L. had strong homoplasy. The phenomenon might be a survival strategy of dioecious plants under long term evolutionary pressure.

    2009年03期 v.10 76-80页 [查看摘要][在线阅读][下载 576K]
    [下载次数:103 ] |[网刊下载次数:0 ] |[引用频次:6 ] |[阅读次数:5 ]

农艺

  • 新育成反向核不育水稻FHS开花习性观察(英文)

    王会峰;欧阳艳蓉;黄群策;

    [Objective] This study was to use reverse genic male sterile FHS better in field production. [Method] Low energy nitrogen ion beam was taken as a mutation source to conduct mutagenic treatment for indica rice 99-02 and the mutant FHS with special fertility that was isolated from their offspring. Some characteristics such as flowering habit and stigma exsertion rate of FHS were observed in this experiment. [Result] The reverse genic sterile rice FHS had an obvious peak flowering stage from 10:00 to 10:30, while the second peak flowering was from August 5 to August 10. Compared with PA64S, FHS flowered early and its flowering time was concentrated, showing that it is for seed propagation. The stigma exsertion rate of FHS was 85.8% and low exsertion rate was good for the purity of seed. [Conclusion] The reverse genic sterile rice FHS had good value in use, besides, it could also be used as comparison material for studying fertility alternation mechanism of photoperiod sensitive genic male sterile rice.

    2009年03期 v.10 81-83页 [查看摘要][在线阅读][下载 280K]
    [下载次数:21 ] |[网刊下载次数:0 ] |[引用频次:9 ] |[阅读次数:6 ]
  • 微胚乳玉米籽粒中淀粉积累规律的研究(英文)

    甘凤琼;张亚平;杨庆锋;吴子恺;

    [Objective] The experiment aimed to study laws of accumulation of starch in micro-endosperm maize kernel. [Method] The laws of accumulation of starch in maize embryo and OSK(the other section of a kernel except embryo) of MEM(micro-endosperm maize) were studied in this experiment by taking Gaoyou 115 maize as control. [Result] The starch accumulation speed of MEM embryo was slightly lower than that of Gaoyou115 and the starch accumulation speed of MEM OSK was far lower than that of Gaoyou 115, so the starch content of the whole kernel of Gaoyou 115 was 3.4 to 4.5 times as high as that of MEM during harvesting. The embryo starch weight ration of Gaoyou 115 maize during harvesting was only 3% to 4% while that of MEM was 16% to 22%. Moreover, starch accumulation speeds of different MEM materials were different and there were significant differences in starch content during harvesting among different MEM materials. [Conclusion] The starch accumulation in MEM was slow, and MEM has widely variation on starch accumulation.

    2009年03期 v.10 84-87页 [查看摘要][在线阅读][下载 480K]
    [下载次数:56 ] |[网刊下载次数:0 ] |[引用频次:8 ] |[阅读次数:5 ]
  • 叶菜型甘薯蔓尖产量构成分析(英文)

    傅玉凡;王卫强;伍加勇;黄世龙;张晓春;高静;张志良;喻淑芬;

    Total yields of vine tip of seven varieties of Leaf-vegetable sweetpotato during 2006-2007 were investigated; proportions of the weights of leaf, leaf stalk and stem in total vine tip yield and their changes among varieties and during topping stages were studied. The results showed that vine tip yields of sweetpotato were significantly different among either varieties or topping stages; leaf yield accounted for about 51% of total vine yield, and changes in leaf yield among topping stages were higher than that among varieties; while yields of leaf stalk and stem each accounted for 25% of total vine tip yield, their changes among varieties were higher than those among topping stages. These results revealed the yield composition of vine tip of Leaf-vegetable sweetpotato, which provided scientific references for breeding and cultivating new Leaf-vegetable sweetpotato variety and its industrialization.

    2009年03期 v.10 88-91页 [查看摘要][在线阅读][下载 237K]
    [下载次数:79 ] |[网刊下载次数:0 ] |[引用频次:8 ] |[阅读次数:10 ]
  • 湿沉降氮对2种禾本科植物中氮含量的影响(英文)

    林菲;史锟;

    [Objective] It was to study nitrogen use efficiency under the condition of deposition of perennial ryegrass (Lolium perenne L.) and barley (Hordeum vulgare L.), further revealing the difference in gene variation.[Method] A pot experiment was conducted under 3 treatments of rainwater, ammonium-N (aN) and nitrate-N (nN).[Result] In the treatments of aN and nN, the biomass and N contents in plants were obviously higher than those in rainwater treatment, while the largest biomass(36.116 g) was observed in nN treatment. The absorption to aN was best to perennial ryegrass while nN to barley. According to the differential analysis of N content, treatments of both aN and nN was nitrogen superfluous, which led to release excessive N to the atmosphere, and the rainwater treatment and the control were N deficient, the largest N absorption from atmosphere was 0.698 g in rainwater treatment.[Conclusion] Average N use efficiency contributed by atmosphere N deposition ranged from 1.321%-6.116%, while the control of barley had the highest of 6.116%.

    2009年03期 v.10 92-96页 [查看摘要][在线阅读][下载 706K]
    [下载次数:60 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:7 ]

动物科学 

  • 水牛子宫内膜腺上皮细胞和基质细胞的分离·培养和生物学特性研究(英文)

    庄新杰;段亚苹;黄怡;宁淑芳;张明;

    [Objective] The experiment aimed to set up a method for isolating and culturing endometrial stromal cells (BESC) and endometrial glandular epithelial cells(BEGEC) of buffalo as well as laid foundation for studying biological mechanism of embryo implantation and uterine diseases. [Method] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique were used to isolate BESC and BEGEC, then immunocytochemical method and TRYPAN-Blue assay were used to determine the purity and survival rate of isolated cells. [Result] The BESC and BEGEC were successfully isolated and cultured while immunocytochemical method and cell count method demonstrated that the purity was over 90%. The result of TRYPAN-Blue assay shown that survival rate of BESC and BEGEC was 91% and 78% respectively. [Conclusion] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique could isolate BESC and BEGEC with high purity.

    2009年03期 v.10 39-42+71页 [查看摘要][在线阅读][下载 435K]
    [下载次数:44 ] |[网刊下载次数:0 ] |[引用频次:7 ] |[阅读次数:9 ]

动物科学

  • 猪繁殖与呼吸综合症GP5蛋白的纯化与免疫活性分析(英文)

    陈妍;田宏;尹双辉;尚佑军;刘湘涛;

    [Objective] The purification and immunocompetence of GPS protein in porcine reproductive and respiratory syndrome (PRRS) were analyzed in this study, which provided basis for establishing the corresponding serological method. [Method] The recombinant expression plasmid pGEX-6P-5 was transformed into BL21 and expressed after being induced with IPTG. The solubility analysis of expression products was carried out, and then the recombinant protein was purified for SDS-PAGE identification and Western-blot analysis. Finally, the recombinant antigen was used in the immune experiment of guinea pigs. [Result] The target protein content accounted for 30% of the total cells protein content according to the chromatography scanning, and the purity of target protein after purification reached 80%. The purified protein was analyzed by Western-blot and immune experiment of guinea pigs, and the results showed that the expressed protein had good reactionogenicity and immunogenicity. [Conclusion] This study provides materials for further studies on the function between PRRSV ORF5 gene and its editing protein, which also lays a foundation for porcine reproductive and respiratory syndrome virus genetic engineering products.

    2009年03期 v.10 97-100页 [查看摘要][在线阅读][下载 331K]
    [下载次数:79 ] |[网刊下载次数:0 ] |[引用频次:4 ] |[阅读次数:8 ]
  • 美凤蝶非滞育蛹·滞育蛹与滞育蛹羽化成虫核酸含量比较(英文)

    易传辉;陈晓鸣;史军义;周成理;

    [Objective] The aim of this study was to provide basis for deeply understanding the diapause mechanism of Papilio memnon L. [Method] RNA and DNA content of non-diapause pupae, diapause pupae and eclosion-adult from diapause pupae at different development stages were detected by the colorimetry. [Result] RNA content of non-diapause pupae was 4.614 0-7.946 3 μg/mg, while diapause pupae was 4.326 0-5.885 3 μg/mg and eclosion-adult from diapause pupae was 20.779 3 μg/mg at initial stage. DNA content of non-diapause pupae was 0.448 7-0.535 0 μg/mg, while diapause pupae was 0.452 0-0.828 3 μg/mg and eclosion-adult from diapause pupae was 1.727 0 μg/mg at initial stage. [Conclusion] The nucleic acid content and change is related to the development stage.

    2009年03期 v.10 101-103+107页 [查看摘要][在线阅读][下载 237K]
    [下载次数:56 ] |[网刊下载次数:0 ] |[引用频次:2 ] |[阅读次数:8 ]
  • 利用Asia 1型口蹄疫病毒VP1蛋白的单克隆抗体建立单抗竞争ELISA方法(英文)

    林彤;邵军军;丛国正;独军政;高闪电;常惠芸;谢庆阁;

    Using the purified VP1 protein of Asia 1 type foot-and-mouth disease virus as the antigen, the purified monoclonal antibody was labeled by the sodium periodate method and the monoclonal antibody competitive ELISA was established in this study. Ten positive porcine foot-and-mouth disease serums and more than two hundreds negative serum were tested, and the results were the same as the background of samples. The sensitivity test and replicate test indicated that this method was stable and sensitive, which was suitable for monitoring Asia 1 type porcine foot-and-mouth disease virus antibody.

    2009年03期 v.10 104-107页 [查看摘要][在线阅读][下载 235K]
    [下载次数:121 ] |[网刊下载次数:0 ] |[引用频次:5 ] |[阅读次数:9 ]

资源与环境

  • 陕蒙高速公路两旁粮食作物中重金属含量分析(英文)

    艾海舰;张雄;刘翠英;纪晓玲;卜耀军;

    To understand the effect of the running of Shaanxi Inner Mongolia Expressway on food crops planted along the sides, we determined the heavy metal contents(Zn, Pb, Cu and Cb) in the digestion solutions of the root, stem and leaf from rice and corn. The results showed that highway promoted the accumulation of heavy metal elements in corn and rice. Compared with the CKs(crops produced where no highway extend), contents of heavy metal elements in two crops tested from two sampling sites were both over standard. This indicates that food crops planted within 100 m away from the highway are been polluted by heavy metals, which is directly related with the running of Shaanxi Inner Mongolia Expressway. Based on this, the areas within 100 m away from the highway are not suggested to cultivate food crops.

    2009年03期 v.10 108-111+119页 [查看摘要][在线阅读][下载 573K]
    [下载次数:94 ] |[网刊下载次数:0 ] |[引用频次:20 ] |[阅读次数:8 ]
  • 池塘底泥营养盐释放的室内模拟研究(英文)

    杜旭彤;谢骏;王广军;余德光;赵旭斌;

    [Objective] The study aimed to research the nutrients release of ponds sediment.[Method] The sediments which from a new pond(A) and an old one (B) these analyses used to carry out indoor experiment under the anaerobic dark condition for researching on nutrient release. The N(nitrogenous) and P(phosphorous) release were analyzed every two days.[Result] At the prophase, the N release in B was bigger than that in A, while the decline sediment release in A was gentle.[Conclusion] There was no accumulation of organic compound during the breeding time. The NH4-N was the main form of N release; and the P release was correlated with N release, while PO4-P was the main form of P release.

    2009年03期 v.10 127-130+138页 [查看摘要][在线阅读][下载 461K]
    [下载次数:141 ] |[网刊下载次数:0 ] |[引用频次:4 ] |[阅读次数:9 ]
  • 基于MODIS/EVI时间序列数据分析冰冻灾害对植被绿叶始期的影响(英文)

    夏浩铭;毕远溥;杨永国;

    In the field of global changes, the relationship between plant phenology and climate, which reflects the response of terrestrial ecosystem to global climate change, has become a key subject that is highly concerned. Using the moderate-resolution imaging spectroradiometer (MODIS)/enhanced vegetation index(EVI) collected every eight days during January- July from 2005 to 2008 and the corresponding remote sensing data as experimental materials, we constructed cloud-free images via the Harmonic analysis of time series (HANTS). The cloud-free images were then treated by dynamic threshold method for obtaining the vegetation phenology in green up period and its distribution pattern. And the distribution pattern between freezing disaster year and normal year were comparatively analyzed for revealing the effect of freezing disaster on vegetation phenology in experimental plot. The result showed that the treated EVI data performed well in monitoring the effect of freezing disaster on vegetation phenology, accurately reflecting the regions suffered from freezing disaster. This result suggests that processing of remote sensing data using HANTS method could well monitor the ecological characteristics of vegetation.

    2009年03期 v.10 131-135页 [查看摘要][在线阅读][下载 610K]
    [下载次数:140 ] |[网刊下载次数:0 ] |[引用频次:7 ] |[阅读次数:6 ]
  • 藏北高原草地生态系统研究进展(英文)

    武建双;沈振西;张宪洲;

    With the aggravation of global change, the response and adaptation of the unique ecosystem in Qinghai-Tibet Plateau to global change have been increasingly concerned by scientific community day by day, which makes the sensitivity and fragility of this ecosystem in response to global change widely recognized by scholars. On the basis of introducing the present research process on the degenerate mechanisim, measures of and approaches to recovery, carbon cycle and primary productivity toward global change, we put forward several propositions on studying the alpine grassland ecosystem in Northern Tibetan Plateau.

    2009年03期 v.10 148-152页 [查看摘要][在线阅读][下载 332K]
    [下载次数:199 ] |[网刊下载次数:0 ] |[引用频次:30 ] |[阅读次数:35 ]
  • 沉湖湿地生物多样性研究(英文)

    宋鄂平;

    Plant diversity, animal diversity and ecosystem diversity in the wetland were studied with field investigation and statistical analysis, and then biodiversity of Chen Lake wetland was also valued. The results showed that the reasonable utilization and protection for biodiversity of Chen Lake wetland gave important contribution to improving the regional environment and sustainable development of Wuhan, and even the middle-lower Yangtze area.

    2009年03期 v.10 153-156页 [查看摘要][在线阅读][下载 218K]
    [下载次数:182 ] |[网刊下载次数:0 ] |[引用频次:17 ] |[阅读次数:3 ]

植物保护

  • β-氨基丁酸诱导水稻稻瘟病抗性对活性氧代谢的影响(英文)

    李莉;郭晓丽;刘晓梅;温嘉伟;张秀容;孙辉;赵宇;任金平;

    [Objective] This study was to understand the effects of β-Amino butyric acid(abbreviated as BABA) induced rice blast resistance on reactive oxygen metabolism. [Method] Using the cultivar Chaochan 2 that is highly susceptible to disease as experimental material, the changes of catalase(CAT), and superoxide dismutase(SOD) and MDA activities in rice treated by BABA were investigated. [Result] In rice plants treated by BABA, the activities of CAT and SOD increased, meanwhile the MDA content also rose to some extent, resulting in the disease resistance to rice blast. [Conclusion] By influencing reactive oxygen metabolism, BABA endows rice plants with resistance to rice blast. BABA is safe to environment and has highly resistance-inducing capacity, it could be generalized in production.

    2009年03期 v.10 112-114页 [查看摘要][在线阅读][下载 335K]
    [下载次数:82 ] |[网刊下载次数:0 ] |[引用频次:11 ] |[阅读次数:11 ]
  • 苜蓿蓟马抗性与生理活性相关性研究(英文)

    刘玉良;米福贵;特木尔布和;王普昶;马小廷;闫蓉;

    [Objective] The experiment explored of resistance of different Medicago sativa through synthetic variations of all physiological indexes and the correlation analysis between physiological activity and damage degree after thrips damage. [Method] According to studying physiological activity (POD activity, SOD activity, PPO activity, PAL activity, MDA content and free proline content) of alfalfa in alfalfa resistance to different number of thrips, the correlation between physiological activity and resistance was investigated. [Result] It was shown that there were negative correlations among POD, SOD, PPO, PAL, MDA, free praline and the number of thrips, and there were negative correlations among POD, SOD, PPO, PAL, MDA, free praline and CAT. The content changes of POD,SOD,PPO,CAT,MDA and free proline in high resistant alfalfa were slow and PAL was quick and the activities of PPO,PAL,CAT were high, and the activities of POD、SOD were low. [Conclusion] The research provided theoretical basis and materials for identifying resistance varieties of alfalfa to thrip.

    2009年03期 v.10 115-119页 [查看摘要][在线阅读][下载 609K]
    [下载次数:102 ] |[网刊下载次数:0 ] |[引用频次:4 ] |[阅读次数:9 ]

贮藏与加工

  • 壳聚糖涂膜对枸杞鲜果常温保鲜的研究(英文)

    李晓莺;何军;葛玉萍;曹有龙;

    [Objective] The aim was to study the preservation of fresh Chinese wolfberry fruit via chitosan coating. [Method] Using Chinese wolfberry "Ningqi No. 1" as experimental material, the fresh fruits were coated by different concentrations of chitosan(0.75%, 1.00%, 1.25%, 1.50% and 1.75%) at room temperature to study the changes in weight loss rate, rate of rotten fruits rot, soluble solid content and vitamin C(Vc) content. [Result] Of all the treatments, the fruits coated with 1.25% chitosan showed lowest rate of rotten fruit and weight loss rate, and highest Vc and soluble solid content, presenting the optimal fresh-keeping effect. [Conclusion] Chitosan coating is helpful for the preservation of fresh Chinese wolfberry fruit at room temperature.

    2009年03期 v.10 120-122页 [查看摘要][在线阅读][下载 307K]
    [下载次数:207 ] |[网刊下载次数:0 ] |[引用频次:11 ] |[阅读次数:7 ]

农业经济

  • 基于灰色关联模型的河南省粮食生产驱动力分析(英文)

    贺振;

    Henan Province is an important grain production base, whose stable production is related to the economic development and social stability significantly. To summarize the primary factors of grain producing, we used grey correlation analysis method to analyze 10 correlative factors such as grain yield, grain yield per unit area, effective irrigated area, rural employed persons, grain planting area, consumption of chemical fertilizer in 25 years. The results showed order of synthetic degree of incidence: effective irrigated area>rural electricity consumption>consumption of chemical fertilizer>disaster crop area>grain yield per unit of area>total power of agricultural machinery>afflicted crop area>grain planting area>number of the rural employed persons. Finally, according to synthetic degree of incidence between influential factors and total grain output respectively, a series of measures and the suggestions was proposed for grain production in Henan Province, which will also provide the scientific basis and help for the government correct decision-making.

    2009年03期 v.10 123-126+147页 [查看摘要][在线阅读][下载 358K]
    [下载次数:102 ] |[网刊下载次数:0 ] |[引用频次:8 ] |[阅读次数:7 ]

药用植物

  • 宁夏枸杞雄性不育育性标准研究(英文)

    曹君迈;任贤;刘建利;贝盏临;宋莎;

    Botanical features of "YX-1" reproductive organ and bearing rate by number of pollen granules in pollen sacs of Lycium chinensis was observed and researched through field investigation, smear preparation and paraffin sections. YX-1 of Lycium chinensis was determined as incomplete male sterility and further classified into complete sterile type, sterile type I and sterile type II. The fertility standard of male sterility of Lycium chinensis was preliminarily proposed to establish a basis for crossbreeding of male sterility of Lycium chinensis.

    2009年03期 v.10 136-138页 [查看摘要][在线阅读][下载 341K]
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