Agricultural Science&Technology

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地址:湖南省长沙市芙蓉区马坡岭省农科院试验大楼湖南省农业信息与工程研究所 Agricultural Science & Technology》(《农业科学与技术》)编辑部(邮编 410125

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农业基础科学与方法

  • 木质素高效降解菌血红密孔菌产酶条件研究(英文)

    王丹梅;任大明;李秀娜;石皎;

    [Objective] The purpose was to study the optimum conditions of Pycnoporus sanguineus for producing ligninase.[Method] A strain of lignin-degrading white-rot fungus was selected from 5 strains of collected fungi and its ligninase production and the optimum conditions for producing ligninolytic enzyme were measured.[Result]It could produce two kinds of ligninase with good thermal stability. Different temperatures, carbon sources, nitrogen sources,acidities, as well as the additions of surfactant had distinct influence on the development of lignin-degrading enzymes of the fungus. The optimum condition was drawn out: 38 ℃, pH=4.5, 10.0 g/L glucose, 1.0 g/L tartaric acid ammonium.[Conclusion] The aim of research was to provide a basis for lignin degradation in practical production.

    2008年01期 1-4页 [查看摘要][在线阅读][下载 437K]
    [下载次数:90 ] |[网刊下载次数:0 ] |[引用频次:10 ] |[阅读次数:5 ]
  • 不同取代度羧甲基壳聚糖的抗氧化性能研究(英文)

    姚倩;孙涛;周冬香;毛芳;

    [Objective] In order to study the relations among different positions,degrees of substitution and antioxidant ability.[Method] N,O-carboxymethyl chitosan(NOA,NOB and NOC)with various degrees of substitution(DS)were obtained by etherizing chito-oligosacchaside.Their structure and substituted degree were characterized and their antioxidant activity to ·OH was evaluated.[Result]The IC50s of NOA,NOB and NOC were 0.15,0.29,0.23 mg/ml while their DSs of-NH2 position(DSN)were 0.51,0.29 and 0.38 and DSO were 0.74,0.84,0.97respectively.[Conclusion]With the increase of DSN,antioxidant activity of N,O-carboxymethyl chitosan oligosaccharide to ·OH was up.

    2008年01期 5-7+59页 [查看摘要][在线阅读][下载 421K]
    [下载次数:132 ] |[网刊下载次数:0 ] |[引用频次:22 ] |[阅读次数:7 ]

植物保护

  • 高山地区3种十字花科蔬菜田节肢动物群落比较研究(英文)

    王香萍;李传仁;王福莲;

    [Objective] This research aimed to compare arthropod communities in different cruciferous vegetable fields at high mountainous area and provide guidance for pest control.[Method] The main arthropod species in 3 kinds of cruciferous vegetable fields at high mountainous area were investigated,the composition of arthropod community in different vegetable fields were analyzed.[Result] The main arthropod species in cruciferous vegetables fields at high mountainous area were similar,but had different quantity compositions.The richness was the highest in radish field.[Conclusion] Crop species had great influence on biological community;we should focus on the main pests in the process of pest control.

    2008年01期 8-10页 [查看摘要][在线阅读][下载 330K]
    [下载次数:33 ] |[网刊下载次数:0 ] |[引用频次:9 ] |[阅读次数:6 ]
  • 蜡样芽孢杆菌JK_(14)对小麦全蚀病的防治及其抑病机理(英文)

    韩艳霞;胡斌杰;王宫南;

    [Objective] Study on control efficacy and inhibitory effect of Bacillus cereus strain JK14 against wheat take-all disease,investigating its antifungal mechanism.[Method] B.cereus JK14 was isolated from soil in the rhizosphere of wheat,inhibitory effects of whose nutrient solution form against Gaeumannomyces graminis var tritici strains 9862 and 9812 were measured in laboratory and then for its antifungal mechanism.The strain JK14 with rifampicin and wheat take-all disease resistance was screened by increasing concentration of the two substrates,and colonization of JK14was studied.[Result] In pot experiment,the control effects of JK14 against 9862 and 9812 are 63% and 59%,respectively,which are higher than that of chemical fungicides,with 55% and 51%,respectively.JK14 could deform mycelium and causes degradation of cell wall.And there are also dynamic change of JK14 in root system.JK14 on seed could extend to root along with seed germination and rooting,but per unit tissue mycelium number decreased gradually.[Conclusion] The results indicate some control efficacy of B.cereus strain JK14 against wheat take-all disease.

    2008年01期 70-74页 [查看摘要][在线阅读][下载 511K]
    [下载次数:94 ] |[网刊下载次数:0 ] |[引用频次:17 ] |[阅读次数:9 ]
  • 稻鸭共育对病虫草害及经济效益的影响(英文)

    邓强辉;潘晓华;

    [Objective] Study on the effects of rice-duck mutualism on weeds and insects pests and economic benefits of paddy field.[Method] Comparison of the incidence of weeds,sheath blight and insects pest under rice-duck mutualism,conventional cultivation and control treatment,the yield and economic benefits were analyzed under the 3 treatments.[Result] Average occurrence of weeds in rice-duck mutualism group decreased by 2.33 and 52.08 ind/m2 compared with that in conventional cultivation and control treatment;the control rate of mutualism was up to 75% against rice hopper,but just between 25%-60% against rice leaf roller and Chilo suppressalis.The rates of diseased plant and diseased holl against rice sheath blight were higher and disease indices were lower compared with control group.The yield of mutualism group was identical with conventional cultivation,which was greatly higher than that of control group.The results suggested an higher economic benefits and lower cost benefit ratio for rice-duck mutualism treatment.[Conclusion] Rice-duck mutualism gives birth to a positive effect to control the diseases,insect pests and weeds,as well as to economic benefits,providing basis on extension of rice-duck mutualism system.

    2008年01期 128-133+138页 [查看摘要][在线阅读][下载 606K]
    [下载次数:88 ] |[网刊下载次数:0 ] |[引用频次:4 ] |[阅读次数:5 ]
  • 小麦多品种混播对条锈病的控制及稳产作用研究(英文)

    沈丽;叶香萍;廖华明;何华健;王胜;刘如东;罗林明;

    Wheat stripe rust has become the most dangerous disease which threaten safe yield of wheat in Sichuan Province.It is meaningful to provide technique support for integrated disease control by exploring the effective control measures of wheat stripe rust.Wheat stripe rust dynamic developments of all-planting and mixed-planting have been systematically investigated in this study by taking different mixed-planting combinations among 6 wheat varieties with different resistance levels.The results of this experiment show that the mixed-plantings of 4 and 6 wheat varieties can delay the occurance of wheat stripe rust,slow the speed of disease and decline the damage of disease as well as stabilize yield of wheats.

    2008年01期 134-138页 [查看摘要][在线阅读][下载 444K]
    [下载次数:56 ] |[网刊下载次数:0 ] |[引用频次:4 ] |[阅读次数:11 ]
  • 臭牡丹叶提取液对2种植物病菌的抑制作用研究(英文)

    尹礼国;林娜;魏琴;张超;周黎军;

    The test was undertaken to reveal the antifungal activity of extracts from Clerodendrum bungei leaves against Pestalotia funereal and Rhizoctonia solani,the results showed that optimal condition for best antifungal activity of extracts against Pestalotia funereal and Rhizoctonia solani are as follows:material-liquid ratio of 1∶6,75% ethanol as extracting solvent,reflux at 90 ℃ for 1.5 h.The substances with good dissolubility in ethanol and water solution such as organic acid,bioflavonoid and alkaloid are main antifungal bioactive substances in Clerodendrum bungei.

    2008年01期 143-145页 [查看摘要][在线阅读][下载 151K]
    [下载次数:97 ] |[网刊下载次数:0 ] |[引用频次:5 ] |[阅读次数:9 ]
  • 链霉素抗性筛选吸水链霉菌高产农用抗生素菌株研究(英文)

    彭祎;黄永春;蔡延明;曹仁林;

    [Objective] The research aimed to screen Streptomyces hygroscopicus strains with high production of agricultural antibiotics.[Method] A strain of S.hygroscopicus was screened from the soil of Hainan Island.After natural screening and consecutive ultraviolet induced mutation twice,S6-7 strain was obtained as the original strain then treated by UV irradiation and streptomycin resistance screening,and finally rescreened through shake-flask fermentation.[Result] 7 better strains were selected by primary screening from 62 single colonies which were picked out randomly.After 3 generations of consecutive cultivation on slant media and rescreening,5 strains presented obvious forward mutation.The forward mutation rate reached 8.06%,and the largest production increasing rate came up to 25.11%.[Conclusion] By combining streptomycin resistance screening and conventional ultraviolet induced mutation,both the antibiotic-producing capacity and forward mutation screening efficiency of the original strain were greatly enhanced.

    2008年01期 146-149页 [查看摘要][在线阅读][下载 273K]
    [下载次数:84 ] |[网刊下载次数:0 ] |[引用频次:12 ] |[阅读次数:8 ]

植物·生理·生化

  • 低温胁迫对甘薯S-腺苷甲硫氨酸合成酶mRNA表达水平的影响(英文)

    岳昌武;肖静;凌锌;曾霓;

    [Objective] The mRNA expression level changes of S-adenosylmethionine synthetase(SAMS)under low temperature stress was studied.[Method]Total RNA were extracted from leaves,stem and earthnut of sweet potato 0,12,24,48 and 72 h after low temperature treatement.mRNA expression level was analyzed by reverse expression and Real-time PCR technique.[Result]The expression quality of the gene extracted from leaves,stem and earthnut increased and the expression quality reached the peak point 24,72 and 72 h after low temperature treatment respectively.The expression change of earthnut was the biggest.[Conclusion]Low temperature was good for increasing mRNA expression of relevart genes.

    2008年01期 11-14+156页 [查看摘要][在线阅读][下载 741K]
    [下载次数:109 ] |[网刊下载次数:0 ] |[引用频次:12 ] |[阅读次数:5 ]
  • 水稻穗上发芽生理生化及颖壳扫描电镜观察(英文)

    蔡建秀;陈伟;

    Scanning electron microscopy of easy or uneasy-germinated glumes,determining the contents of endogenous hormones and the activity of α-amylase were studied from at 1-4 d pre-harvest sprouting rice.The results showed that the activity of α-amylase and the contents of IAA,GA1+3 and ZR were higher in easy-germinated rice than those in the uneasy-germinated rice,while ABA content in easy-germinated rice was lower than that in uneasy-germinated rice.The change of GA1+3/ABA rate accorded with that of GA1+3.Scanning electron microscopy showed that the cell surface of glume was loose arranged with more villus and bigger hole and germinal aperture,which had lesser mechanism resistance and better water absorptivity in easy-germinated rice.This research also indicated that pre-harvest sprouting in rice related to α-amylase activity,endogenous hormone contents and glume structure.

    2008年01期 75-80页 [查看摘要][在线阅读][下载 840K]
    [下载次数:126 ] |[网刊下载次数:0 ] |[引用频次:9 ] |[阅读次数:10 ]
  • 蒜氨酸酶动力学性质研究(英文)

    葛艳辉;赵俊英;闵笛;冯炘;

    [Objective] The kinetic characteristics of alliinase was studied to select the optimum reaction performance.[Method] Alliinase activity was measured to analysis the influence of temperature,pH,substrate concentration and metal iron.[Result] Alliinase was an enzyme with thermal instability.Its optimum reaction temperature was 29 ℃ and pH value was 6.1.The Vmax was 0.439 IU/mg and Km was 0.483 mmol/L by using natural extract as substrate.Alliinase activity was activated when the K+,Mg2+,Na+ and Cd2+ existed and alliinase activity was inhibited when Cu2+ existed.[Conclusion] Results showed that the kinetic characteristics of alliinase supply the academic foundation for development and application of garlic medical products.

    2008年01期 139-142页 [查看摘要][在线阅读][下载 665K]
    [下载次数:85 ] |[网刊下载次数:0 ] |[引用频次:5 ] |[阅读次数:4 ]

农业生物技术

  • 禽流感病毒H5亚型及H7亚型基因的引物设计与多重RT-PCR扩增(英文)

    张文慧;郭华;王伟利;刘明;钱爱东;

    [Objective] The research aimed to design primers that are suitable for detecting H5 and H7 subtypes of avian influenza virus(AIV);[Method] DNAStar was used to analyze the homology of the sequences of H5 and H7 subtypes of AIV accessed in GenBank,and design primers(by Primer Premier 5.0)on high homologous region of these sequences,and then amplified by RT-PCR.[Result] The multiplex RT-PCR amplification,agarose gel electrophoresis and sequencing results showed that the self-designed primers are successful for detecting AIV.[Conclusion] It is feasible to rapidly diagnose AIV through this method.

    2008年01期 15-17页 [查看摘要][在线阅读][下载 430K]
    [下载次数:170 ] |[网刊下载次数:0 ] |[引用频次:9 ] |[阅读次数:10 ]
  • 野百合试管鳞茎诱导与增殖的研究(英文)

    刘红美;令狐克勇;方小波;

    With different parts of bulb scale as explants,the proliferation method of Guizhou Lilium brownii were studied with 3% sodium hypochlorite and MS medium with different concentrations of hormones.The results show that it is feasible to disinfect the bulbs of Lilium brownii with 3% sodium hypochlorite,moreover,the sodium hypochlorite is very cheap and harmless to researchers,experimental materials and environment.MS+NAA 0.3 mg/L+6-BA 1.5 mg/L is optimum for the induction of bulbs and,the basal part of Lilium brownie is the optimum explants.After culture for 25 d on the same medium,the tube bulbs could be obtained with the characteristics of high propagation coefficient,strong and new roots.The survival rate is over 90% for transplantation of tube bulbs with diameter between 1-2 cm.The method developed in the present study can proliferate abundant Lilium brownii seedling in short time.

    2008年01期 18-20+53页 [查看摘要][在线阅读][下载 220K]
    [下载次数:83 ] |[网刊下载次数:0 ] |[引用频次:27 ] |[阅读次数:9 ]
  • 芨芨草遗传多样性研究(英文)

    徐海霞;张霞;王绍明;阎平;杜金洲;

    [Objective] Study on the genetic diversities of A.splendens from different areas of xinjiang.[Method] The genetic diversities among seven populations and within two populations were analyzed by RAPD.[Result] Genetic clustering results presented that the relationships among populations of A.splendens are directly related with geographic positions,and within population are related with habitats.[Conclusion] RAPD technique can be used to study genetic diversity of A.splendens.

    2008年01期 21-23+28页 [查看摘要][在线阅读][下载 436K]
    [下载次数:57 ] |[网刊下载次数:0 ] |[引用频次:5 ] |[阅读次数:13 ]
  • 应用RACE法分离和克隆猪GPX2基因研究(英文)

    赵华;周继昌;李俊刚;赵莹;王康宁;

    [Objective] Using molecular biotechnology to clone the sus scrofa GPX2 gene.[Method] Using total RNA of sus scrofa duodenum as template,degenerated primer pairs were designed according to the homology alignment analysis of GPX2 gene of human,rat,mouse,dog and cattle.A sus scrofa GPX2 gene sequence of 330 bp was obtained by RT-PCR application method.Primes were designed respectively according to the known sequence,sus scrofa GPX2 gene was isolated and cloned by 3-RACE and 5-RACE method and analyzed the gene sequence.[Result] A mRNA sequence of 924 bp was successfully cloned and isolated in this research.This sequence contained complete 3' end and had higher sequence homology with human,mouse,cattle and dog GPX2 gene,and there was codon called TGA which encoding Sec on the position of No.114-116 gene.[Conclusion] Sequence alignment analysis showed that the cloned gene was sus scrofa GPX2 gene(NCBI GenBank database,the sequence number was DQ98982).

    2008年01期 24-28页 [查看摘要][在线阅读][下载 1413K]
    [下载次数:93 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:5 ]
  • 一种有效提取无核荔枝幼胚总RNA的方法(英文)

    刘兴地;郑学勤;

    A total RNA extraction method for young embryo of seedless litchi was introduced.CTAB,Phenol(saturated with water),chloroform,Guanidine isothiocyanate were used as main extraction reagents.Polyphenolic compounds were removed effectively by added PVP into the extraction buffer solution.RNA was purified intensively by phenol,chloroform extraction,and ethanol deposition after deposited by LiCl.Both the results of formaldehyde denatured agarose gel electrophoresis and ultraviolet spectrophotometer analysis showed high integrity and purity of RNA.So the quality of extracted RNA could meet the demand of most molecular biology experiments that require higher quality RNA.

    2008年01期 29-31页 [查看摘要][在线阅读][下载 404K]
    [下载次数:50 ] |[网刊下载次数:0 ] |[引用频次:13 ] |[阅读次数:8 ]
  • Peat1蛋白及其3个缺失突变体的表达与热稳定性分析(英文)

    李明勇;邱德文;曾洪梅;杨秀芬;

    [Objective] The research aimed to reveal the functions of NAC and UBA domains in Peat1's thermal stability.[Method] Fusion expression vectors of Peat1 protein and the 3 deletion mutants were constructed.The recombinant plasmids were induced by IPTG and the target proteins(Peat1,Peat1-△CD99,Peat1-△ND49 and Peat1-△ND108)were expressed obtained by AKTA and its thermal stability was analyzed.[Result] The research found that 3 deletion mutants have good thermal stability like Peat1.[Conclusion] The research demonstrated that the coexistence of NAC or UBA domains is not necessary to thermal stability of Peat1 protein,and they may give the protein particular stability structure seperately.

    2008年01期 32-34+38页 [查看摘要][在线阅读][下载 503K]
    [下载次数:34 ] |[网刊下载次数:0 ] |[引用频次:3 ] |[阅读次数:13 ]
  • 枸杞nrDNA ITS测序鉴定的初步研究(英文)

    石志刚;安巍;樊云芳;焦恩宁;赵建华;王亚军;

    [Objective] The study aimed to identify wolfberry(Lycium Linn.)germplasm resources at molecular level by analyzing the nrDNA ITS sequence.[Method] Genomic DNA from wolfberry leaves extracted by modified CTAB method were regarded as templates for PCR amplification by specific primer,clone and sequencing.[Result] The nrDNA ITS sequences were obtained and then differentiated among three tested materials.[Conclusion] PCR amplification and sequencing on nrDNA ITS is a feasible approach to identify different wolfberry germplasm resources.

    2008年01期 35-38页 [查看摘要][在线阅读][下载 505K]
    [下载次数:96 ] |[网刊下载次数:0 ] |[引用频次:9 ] |[阅读次数:6 ]
  • 抗A型禽流感病毒核蛋白特异性单克隆抗体研究(英文)

    李娜;秦爱建;邵红霞;金文杰;刘岳龙;

    Five monoclonal antibodies(Mabs)to nuclear protein of avain influenza virus(AIV)were developed by syncretizing SP 2/0 and the spleen cells from BALB of mice immuized with H9 subtype AIV.Specificity of these Mabs were identified by immunofluorescent assay(IFA)and enzyme linked immunosorbent assay(ELISA).These five Mabs which were named as AIV-NP-2C3,AIV-NP-6A5,AIV-NP-3H9,AIV-NP-7B4,AIV-NP-2H4 could react with all viruses of AIV-H9 strains in tests.The result of Western blotting showed that only the 60 ku protein antigen of AIV-H9 could be recognized by the Mabs but never recognized by New castle disease virus,REV and infectious bursa disease virus.The result of preliminary application showed that avian influenza viruses could be dectected by Mabs in IFA and ELISA.All these Mabs will probably play important roles in preventing and monitoring avian influenza viruses.

    2008年01期 60-63+66页 [查看摘要][在线阅读][下载 437K]
    [下载次数:69 ] |[网刊下载次数:0 ] |[引用频次:9 ] |[阅读次数:8 ]
  • 极细链格孢菌蛋白激发子PeaT1在酵母双杂交系统中转录激活活性的检测(英文)

    刘延锋;邱德文;曾洪梅;杨秀芬;

    The peaT1 gene fragment was amplified from pGEM-6p-1-peaT1 by PCR,and recovered target gene was cloned into pLexA vector.After digestion and sequencing,the bait vector pLexA-peaT1 was transformed into yeast strain EGY48 [p8op-lacZ] by PEG/LiAC,and the transcriptional activity of bait vector was detected.The results showed that recombinant bait plasmid pLexA-PEMG1 was constructed,for the two bands of recombinant bait plasmid in agarose gel electrophoresis were expected after digesting by restriction endonuclease EcoR I and Xho I.Therefore,the recombinant bait plasmid could be used in yeast two-hybrid system to screen a cDNA library.

    2008年01期 64-66页 [查看摘要][在线阅读][下载 224K]
    [下载次数:52 ] |[网刊下载次数:0 ] |[引用频次:3 ] |[阅读次数:11 ]

农艺

  • 内生菌拟茎点霉B3促进大田水稻增产的初步研究(英文)

    戴传超;袁志林;杨启银;史青山;李霞;

    After insufflating extended endophytes phomopsis B3 ferment diluent on seedling bed of rice,the effect of microbial inoculum on rice growth was studied.The investigation result of seedlings before transplanting seedling showed that leaf age,the number of green leaves,the number of tiller of individual plant and the weight of per 100 fresh plants in microbial inoculum treatment group increased 0.11,0.12,0.03 and 2.6 g respectively compared with those of control group.20 days after transplanting,increased tiller number in microbial inoculum treatment group was 55 500 hm2 more than that in control group.30 days after transplanting,tiller number in microbial inoculum treatment group was 42 000 hm2 more than that in control group.In harvest season,the yield,weight of 1 000 grain and seed-setting rate increased 300 kg/hm2,0.7 g and 0.23% respectively compared with these in control group.The result of preliminary study demonstrated that microbial inoculum confected by endophytes phomopsis B3 could increase the number of strong seedling and yield.

    2008年01期 39-42+48页 [查看摘要][在线阅读][下载 528K]
    [下载次数:174 ] |[网刊下载次数:0 ] |[引用频次:24 ] |[阅读次数:69 ]
  • 稻种资源低温贮藏生活力跟踪监测与种质更新(英文)

    宁秀呈;

    53 rice germplasm resources warehoused during 1981-1984 were regarded as materials to monitor the viability at warehouse time and different years after warehoused.The results showed that seed germination rates of different rice germplasm resources assumed descending trend in storage,with annual decreasing rate between 0.12%-3.05%;the seed germination rates of most cultivars were above 75% after stored for 26 years;forecasting analysis based on the germination rate of 75% as reference showed a huge difference of safe storage life for different rice germplasm resources,ranging from 12 to 50 years,even longer time.The results suggest that track monitoring on viability and regeneration of rice cultivars is of great importance for germplasm resources conservation.

    2008年01期 43-48页 [查看摘要][在线阅读][下载 396K]
    [下载次数:13 ] |[网刊下载次数:0 ] |[引用频次:3 ] |[阅读次数:6 ]
  • 甘蓝型油菜新不育系恢复材料花药培养影响因素的研究(英文)

    李浩杰;蒲晓斌;张锦芳;张启行;蒋梁材;

    Some influential factors of anther culture were studied preliminarily by conducting anther culture of the restorers of new cytoplasmic male sterile(NER).Several results were obtain from this experiment and they were listed as follow:① MS cultrure medium with such hormones as 2,4-D 2 mg/L,6-BA 0.5 mg/L,NAA 0.5 mg/L was the best suitable for callus induction of NER.②The difference of induction rate was significantly different between different plant age groups.From the 110th day to 141th day,the induction rate was increased with the increase of age and the difference of induction rate reached 0.01 significant difference level.The induction rate reached the highest value in the 141th day then it declined gradually.③The combined use of 2,4-D and 6-BA with proper increase of 2,4-D was good for inducing callus.④The green plantlet induction rate of NER was increased when the concentration of 6-BA increased from 2 mg/L to 4 mg/L.Adding ZT from 0.5 mg/L to 2 mg/L,6-BA would led 2.47% increase of green plantlet induction rate.

    2008年01期 49-53页 [查看摘要][在线阅读][下载 308K]
    [下载次数:41 ] |[网刊下载次数:0 ] |[引用频次:13 ] |[阅读次数:13 ]
  • 甘蔗茎尖细胞有丝分裂过程中微管骨架的变化(英文)

    李志刚;赵洪波;李素丽;杨丽涛;李杨瑞;

    In order to understand the microtubule change of monocotyls stem-tip during mitosis,the arrangement,transformation of microtubule array and its relation with chromosome movement during mitosis were studied with freezing microtome,indirect immunofluorescence,DAPI staining and fluorescence microscopy.The results showed that nucleolus was intact when the cortical microtubules formed;cortical microtubules were changed into phramoplast microtubule bands at mitosis prophase.When phramoplast microtubule came into being,nuclear membrane was ruptured and chromosome was arranged at the position of cell plate;subsequently,phramoplast microtubules were changed into phragmoplast microtubules,phramoplast microtubules were shortening and microtubules on the sides of cell plate were increasing gradually,during this course sister chromatid was separated by microtubules at cell plate and tract to the two poles,forming phragmoplast microtubules.Then the nucleolus of two daughter cells formed and separated in the end with the increase of cells numbers.Therefore,cell division orientation could be judged from the arrangement of cell microtubules in different periods in order to understand its growth status.

    2008年01期 94-98+149页 [查看摘要][在线阅读][下载 648K]
    [下载次数:68 ] |[网刊下载次数:0 ] |[引用频次:14 ] |[阅读次数:9 ]
  • 苯达松敏感基因在杂交水稻制种技术上的应用研究(英文)

    王士梅;朱启升;汪婉琳;张德文;杨前进;王云生;

    The seed production of male and female parent of 'Hunzhi 1' contained bentazon susceptible gene was studied.The result showed that spraying 3.375 L/hm2 48% bentazon aqua could control the self-pollinated fertilization rate below 1.0% and survival plant rate was 0.The spray of bentazon would not influence MC526 pollen vitality.Seed setting rate of female parent was not obvious different from that of control group with spraying bentazon aqua more than 3.375 L/hm2 from the sprouting appearing period to full heading stage.This demonstrated that bentazon aqua had no influence on seed setting rate during florescence of female parent.The weight ratio between male parent seeds and female parent seeds was 1 to 5,then seeds from parents with that weight ratio would be mixedly planted for seed production.With this weight ratio,relative proper structure of panicle and grain would be obtained,panicle ratio was 1 to 4.3,glumous flowers ratio was 1 to 3.5.Cross seed-setting rate of female parent was 72.6% and seed production per hm2 was 66 000 kg.

    2008年01期 99-103+145页 [查看摘要][在线阅读][下载 392K]
    [下载次数:65 ] |[网刊下载次数:0 ] |[引用频次:12 ] |[阅读次数:31 ]

园林·园艺

  • 玉兰属植物特异特征的新发现(英文)

    赵东欣;赵东武;孙军;

    The research history and new discoveries of the specific characteristics of Yulania Spach were reported,as to the specific characteristics,such as the terminal,axillary and fascicled similar flower buds,sometimes polycymes;2-4 flower types of a plant;two spathe-like stipules of single flower;drape or overlap of tepals,deep split or shallow split on the edge of tepals.The discoveries could provide valuable information for the further studies on the taxonomic system,anthotaxical evolution,phylogenetic relationship and breeding.

    2008年01期 54-59页 [查看摘要][在线阅读][下载 522K]
    [下载次数:36 ] |[网刊下载次数:0 ] |[引用频次:2 ] |[阅读次数:5 ]
  • 冬凤兰非共生萌发和低温离体保存(英文)

    罗远华;冷青云;莫饶;陈业渊;

    [Objective] The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro.[Method] With mature seeds of C.dayanum as explants,different media were selected to establish asymbiotic germination technique system.With protocorms as materials,conservation,resumptive proliferation and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily.[Result] Mature seeds of C.dayanum could germinate after cultured 90 days on MS media as well as "Hyponex 1" media.The germination rate reached more than 98%.Protocorms inoculated in "Hyponex 1" media could be conserved continuously at 5 ℃ in dark for more than 18 months and the survival rate could reach 90%.Conserved protocorms could realize resumptive proliferation culture both on 1/2 MS and "Hyponex 1" media.The seedling-strengthening and rooting media were 1/2 MS media.[Conclusion] This research provided practical basis for in vitro conservation and rapid propagation of C.dayanum germplasm resource.

    2008年01期 67-69+74页 [查看摘要][在线阅读][下载 403K]
    [下载次数:61 ] |[网刊下载次数:0 ] |[引用频次:2 ] |[阅读次数:6 ]

动物科学

  • GABAergic神经元在BARREL和VPM区组织结构及形态特点的研究(英文)

    范增杰;曹志恒;王子仁;

    To understand the structure of GABAergic neurons in the VMP and "barrel",the distribution of GABAergic neurons in the two areas were studied through immunohistochemistry and Laser Scanning Confocal Microscope.The results show that the distribution of GABAergic neurons in VMP and barrel are different,and the coding of information transmission in the two areas are also dissimilar;GABAergic neurons mainly distribute among the lines asymmetrically in VMP,the somata,dendrite and axon of GABAergic neurons are restricted in the "barrel",rarely having synaptic connections with other "barrel" around.Therefore,VMP and barrel may have different roles in transmission and on processing of informatiton.

    2008年01期 81-84页 [查看摘要][在线阅读][下载 486K]
    [下载次数:26 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:9 ]
  • 培养条件对奶牛金葡菌5型荚膜多糖产量的影响(英文)

    杨正涛;张乃生;刘庆涛;杨琦;尹荣兰;

    [Objective] The effect of different culture conditions on type 5 capsular polysaccharide production of Staphylococcus aureus from diary cattle was studied to provide simple way for CP production and preparation and laid foundation for carrying out new polysaccharide vaccine research.[Method] Staphylococcus aureus was isolated from milk sample of sick dairy cattle and capsular polysaccharide serotypes were identified.Type 5 capsular polysaccharide was cultured on BHI,solid columbia and mod110 culture media.Glucose and lactose were taken as carbon sources for every culture media in solid and liquid state.Therefore 9 different culture conditions were taken to study the effect of culture conditions on capsular polysaccharide production.[Result]Different culture conditions indicated that compared with columbia culture media,BHI culture media could decline capsular polysaccharide production and mod110 culture media could increase capsular polysaccharide production.While for same culture media,solid culture media was better for capsular polysaccharide production,meanwhile,taken lactose as carbon source could increase capsular polysaccharide production.

    2008年01期 85-88页 [查看摘要][在线阅读][下载 361K]
    [下载次数:57 ] |[网刊下载次数:0 ] |[引用频次:11 ] |[阅读次数:7 ]
  • 非洲猪瘟病毒VP73蛋白的B细胞表位预测(英文)

    李倩;姚淑霞;

    [Objective] The B cell epitopes for VP73 protein of African swine fever virus was predicted.[Method]Based on the analysis of the amino acid sequence and the flexible regions of VP73 protein,the B cell epitopes for VP73 protein of African swine fever virus were predicted by method of Kyte-Doolittle,Emini and Jameson-Wolf.[Result]The B cell epitopes were located at or adjacent to the N-terminal No.11-18,26-48,73-82,136-150,159-174,181-189,191-210,247-276,279-295,313-323 and 382-392.[Conclusion]The multi-parameters analytic method was adopted to predict the B cell epitopes for VP73 protein of African swine fever virus,which laid solid foundation for further characterizing the protein of VP73 and researching epitope vaccine.

    2008年01期 89-93页 [查看摘要][在线阅读][下载 612K]
    [下载次数:92 ] |[网刊下载次数:0 ] |[引用频次:7 ] |[阅读次数:8 ]
  • 东北林蛙8个地理种群的形态聚类分析(英文)

    应璐;徐艳春;黄孝明;田秀华;汪青雄;

    [Objective] The research aimed to determine the geographic distribution map of system of Rana dybowskii.[Method] Four morphologic indices(body length,body weight,forelimb length,hindlimb length)of eight geographical populations of R.dybowskii which naturally distribute in Changbai Mountain and Xiaoxing'an Mountain were measured.Measure results were variance analyzed and cluster analyzed.[Result] Variance analysis showed:the genetic branching among the Dongfanghong male population(belongs to Wandashan)and Xiaoxing'an Mountain male population and Changbai Mountain male population were significantly different(P<0.05);the genetic branching between the Hebei female population(belongs to Xiaoxing'an Mountain)and Changbai Mountain female population was significantly different(P<0.05).Cluster analysis showed:male R.dybowskii can be divided into three groups:the first group included Quanyang,Tianbei,Chaoyang and Dakouqin,the second group included Tieli and Anshan,the third group included Dongfanghong;and the female R.dybowskii can be divided into three groups:the first group included Quanyang and Chaoyang,the second group included Tianbei and Dakouqin,the third group included Hebei.[Conclusion] The paper deduced that the Sanjiang Plain was the geographical origin center of R.dybowskii which radiated to Changbai Mountain and Xiaoxing'an Mountain along the adverse current of Songhua River basin,therefore,the current distribution pattern of R.dybowskii was formed.

    2008年01期 104-106+110页 [查看摘要][在线阅读][下载 304K]
    [下载次数:112 ] |[网刊下载次数:0 ] |[引用频次:4 ] |[阅读次数:5 ]
  • 代乳料对羔羊生产性能及血液生化指标的影响(英文)

    郭爱伟;张力;熊春梅;周学辉;苗小林;

    [Objective] In this study,the performance and blood biochemical indices in lamps fed on milk replacer were researched,the feasibility of early weaned in lamps was discussed.[Method] 37 of 10 days lambs of Suffolk(♂)×XiaoWeiHanYang(♀)were randomly divided into group A,B,C,D for this experiment.Group A,B,C were fed by 3 milk-replacers containing milk for different protein source respectively,and ten lambs in each group;group D(seven lambs)were fed by ewes as the control.The performance and blood biochemical indices of the lamps were determined.[Result] The results showed that the average daily gains of 10-15 days of age was not significantly different between group A and D(P>0.05),the control were higher significantly than the group B,C(P<0.05).The average daily gains of 16-25,26-35 days of age were not significantly different in group A,B,C,D.The daily weight gains of Group A,B,C in 45-55 days of age were higher significantly than the control(P<0.05).Plasma urea nitrogen(PUN)of group B and C were higher than group A significantly(P<0.05);Total protein(TP),Plasma albumin(ALB)and Plasma total amino acid were no significant difference among the other 3 groups(P>0.05).[Conclusion] So,the milk-replacers were available for early-weaned lamb.

    2008年01期 107-110页 [查看摘要][在线阅读][下载 238K]
    [下载次数:65 ] |[网刊下载次数:0 ] |[引用频次:9 ] |[阅读次数:9 ]
  • 商城肥鲵的含肉率及其肌肉生化成分分析(英文)

    彭仁海;张丽霞;

    [Objective] The ratio of flesh content and biochemical compositions in muscles of Pachyhynobius shangchengensis were researched in this experiment to provide basic referrences for studying nutritive physiological ecology of Pachyhynobius shangchengensis.[Method]The biochemical analytic method was used to detect the ratio of flesh content and biochemical compositions in muscle of Pachyhynobius shangchengensis.[Result]The ratio of flesh contents of female and male Pachyhynobius shangchengensis were 54.93% and 55.84% respectively.The contents of protein,total saccharides and fatty acid of female Pachyhynobius shangchengensis were 15.44%,1.70% and 0.68% respectively,while the same contents of male Pachyhynobius shangchengensis were 15.67%,1.89% and 0.53% respectively.The total content of 18 kinds of amino acids was 12.72%(the proportion in fresh weight),among which the content of 8 kinds of essential amino acids was 4.68%.The essential amino acids index(EAAI)was 72.68 and limiting amino acids was valine.There were 11 kinds of fatty acids in muscles of Pachyhynobius shangchengensis,among which the content of unsaturated fatty acids(80.02%)was higher than that of saturated fatty acids(19.98%).[Conclusion] Pachyhynobius shangchengensis was a kind of valuable natural resource belonging to the nutritious type of low fat content and high protein content.

    2008年01期 111-116+142页 [查看摘要][在线阅读][下载 587K]
    [下载次数:47 ] |[网刊下载次数:0 ] |[引用频次:3 ] |[阅读次数:5 ]
  • 洋葱对草鱼细菌性烂鳃病的治疗效果试验(英文)

    徐大勇;韦汉群;张文丽;

    [Objective] The curative effect of onion on bacterial rotted gill disease in grass carp was researched[Method]The combination method of taking medicine through oral and spraying was used to cure sick grass carp for 1 period of treatment in room under artificial conditions.[Result]Different concentrations of onion generated different cure rates.When the combination was adding 1.0%-2.0% medicine into feed and spraying 2.0 g/m3-5.0 g/m3,the curative result was the best with cure rate was 70%-90%[Conclusion]The onion was effective on curing bacterial rotted gill disease in grass carp and could be taken as curative medicine.

    2008年01期 117-120页 [查看摘要][在线阅读][下载 364K]
    [下载次数:54 ] |[网刊下载次数:0 ] |[引用频次:4 ] |[阅读次数:8 ]
  • 紫苏叶提取物对高脂血症家兔血脂及脂质过氧化的影响(英文)

    谭健民;陈眷华;徐在品;霍欣;邓小燕;张迪;简秀华;

    [Objective] The preventive and therapeutic effects of Folium perillae extract(FPE)on hyperlipemia induced by feeding rabbit with high fat forage were observed.[Method] The high fat and cholesterol diet were adopted to create the hyperlipemia model of rabbit.The rabbits were randomly and equally divided into 5 groups which were NC(normal control)group,HLM(high lipid model)group,LFPE,MFPE and HFPE group(low,middle,high dosage of FPE were 0.17 g/kg,0.5 g/kg and 1.5 g/kg respectively).The contents of TC,TG,LDL-C,HDL-C,MDA and the activity of SOD in serum were detected respectively.[Result] The three FPE groups could decrease the contents of TC,TG,LDL-C,MDA significantly(P<0.01)compared with these of HLM group,and the contents of HDL-C(P<0.01))and the activity of SOD(P<0.05)in serum was increased significantly.[Conclusion] FPE could regulate blood-fat and counteract lipid peroxidation.

    2008年01期 121-124页 [查看摘要][在线阅读][下载 236K]
    [下载次数:75 ] |[网刊下载次数:0 ] |[引用频次:12 ] |[阅读次数:12 ]

药用植物

  • 4种类型茅苍术生长的比较(英文)

    桑小花;吴沿友;赵玉国;许梦云;

    [Objective] The research aimed to compare the photosynthetic rates and growth conditions of four types of Atractylodes Lancea(Thunb.)DC..[Method] A.Lancea was divided into four types:the saw-tooth-leaf,the ellipse-leaf,the long-ellipse-leaf as well as the sharp-leaf,according to leaf morphology.The photosynthetic rates of four types of A.Lancea were determined by the portable photosynthetic instrument LI-6400 in natural environment.The parameters such as stem width,stem height,leaf area of single plant of aboveground parts were measured periodically.[Result] The photosynthetic rates of four types of A.Lancea were:the saw-tooth-leaf 20.2 μmol/(m2·s),the ellipse-leaf 18.96 μmol/(m2·s),the long-ellipse-leaf 15.6 μmol/(m2·s)and the sharp-leaf 17.6 μmol/(m2·s),respectively.The photosynthetic rate of the saw-tooth-leaf was higher than the other three types.The growth rate of the saw-tooth-leaf was the highest in the four types at 0.01 level.[Conclusion] The research provided certain theoretic basis for further research on A.Lancea.

    2008年01期 125-127页 [查看摘要][在线阅读][下载 174K]
    [下载次数:80 ] |[网刊下载次数:0 ] |[引用频次:4 ] |[阅读次数:7 ]

资源与环境

  • 我国夏季降水类型初探(英文)

    李鸾;

    [Objective] Study on the spatial distribution of summer precipitation patterns and interannual and interdecadal variability.[Method] The summer precipitation patterns were obtained from standard field of summer precipitation data for 160 observation stations in China during 1951-2000 by the utilization of empirical orthogonal function(EOF),and characteristics of interannual and interdecadal variability were analyzed.[Result] The summer precipitation mainly distributes in eastern part of China;The 1st,2nd and 3rd EOF modes of spatial distribution are especially remarkable as well consistent with the results of previous reports about three rainfall patterns from analysis on the percentages of precipitation anomaly of summer.[Conclusion] There exists interannual and interdecadal variability for summer precipitation in China.

    2008年01期 150-156页 [查看摘要][在线阅读][下载 1876K]
    [下载次数:61 ] |[网刊下载次数:0 ] |[引用频次:3 ] |[阅读次数:4 ]

  • 投稿须知

    2008年01期 157页 [查看摘要][在线阅读][下载 465K]
    [下载次数:22 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:4 ]
  • 卷首语

    2008年01期 158页 [查看摘要][在线阅读][下载 387K]
    [下载次数:4 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:3 ]
  • The Periodical is Embodied by BIOSIS Database

    <正>As the largest database covering the biological abstract and index developed by Biosciences Information Service of Biological Abstracts(BIOSIS),BIOSIS Previews(BP)monitors more than5,500 serials and represents more than 1650 symposia and reports from over 100 countries.BP provides an expansive index to life sciences and

    2008年01期 17页 [查看摘要][在线阅读][下载 265K]
    [下载次数:7 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:6 ]
  • Instructions for Authors

    <正>Titles The most important and specific content should be reflected by the most proper and concise words.Subtitle(second-grade title)is not used usually in this journal.Title of the manuscript should be within20words and avoid uncommon abbreviation,abbreviation of the first letter,character

    2008年01期 31页 [查看摘要][在线阅读][下载 158K]
    [下载次数:4 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:4 ]
  • The Periodical is embodied by CAB Abstract Database

    <正>Centre for Agriculture and Bioscience International (CABI)is an international Agricultural information Institute with headquarters in Britain.CAB Abstracts is an abstract disc database that has been published by CABI since1989.In database,all records are col

    2008年01期 41页 [查看摘要][在线阅读][下载 190K]
    [下载次数:4 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:4 ]
  • 下载本期数据